Brief Introduction
Internal combustion engines before they are used. They consist of Used engine oil is defined as used lubricant oils removed from the crankcase of hydrocarbons (80-90%) and performance enhancing additive (10-20%)
engines oils are altered during use by vehicles, motor bikes, generators, and other machinery, because of the breakdown of additives contamination with the product of combustion and the addition of metals from the wear and tear of the engine.
It is recognized that the major component consisting of aliphatic and aromatic hydrocarbons such as phenol,naphthalene,benzene,(a) antracene,benzo (a)preen, fluranthene,lead, cadmium, and other potentially toxic metals (Jain et al..2009)
Used motor oil can cause great damage to sensitive environments and soil microorganisms substantial volumes of soil have been contaminated by used oil in many countries of the world especially industrialized nations, high
concentration of aliphatic, polycyclic, aromatic, hydrocarbon and heavy metals contribute to the inherent toxicity of used engine oil (Vasquez and bertha 1989) large amounts of used engine oil are liberated in to the
environment.
Table of Contents
Title Page– — — — — — — — — — — –i
Certification– — — — — — — — — — –ii
Dedication—- — — — — — — — — — –iii
Acknowledgement– — — — — — — — — –iv
Abstract —- — — — — — — — — — — v
Table of Content — — — — — — — — — –vi
CHAPTER ONE
1.0 Introduction– — — — — — — — — — –1
1.1Statement of the Problem– — — — — — — — — 2
1.2 Aims and Objectives– — — — — — — — –3
CHAPTER TWO
2.0 Literature Review– — — — — — — — — -4
2.1 Soil and its its Microbial Flora– — — — — — — — 4
2.2 Effects of Petroleum Hydrocarbons– — — — — — — 5
CHAPTER THREE
3.0 Materials and Method — — — — — — — — 10
3.1 Experimental site — — — — — — — — 10
3.2 Sample Collection — — — — — — — — 10
3.3 Sterilization — — — — — — — — — — — 10
3.4 Experimental Procedure– — — — — — — — 11
3.4.1 Preparation of Soil Dilution– — — — — — — 11
3.4.2 Experimental Procedure in Gram Staining– — — — — 11
CHAPTER FOUR
4.0 Results and Discussion– — — — — — — — 13
4.1 Results– — — — — — — — — — — 13
4.2 Discussion– — — — — — — — — — 19
CHAPTER FIVE
5.0 Conclusion, Recommendation and Summary– — — — –21
5.1 Conclusion– — — — — — — — — — –21
5.2 Recommendation– — — — — — — — — –21
5.3 Summary– — — — — — — — — — –22
REFERENCES– — — — — — — — — — –23
